Natural Occurrence of Cross-Linked Vaccinia Virus Deoxyribonucleic Acid
نویسندگان
چکیده
منابع مشابه
Natural occurrence of cross-linked vaccinia virus deoxyribonucleic acid.
The molecular weight of native vaccinia deoxyribonucleic acid (DNA) is 1 to 1.17 times that of native T4 DNA. Sedimentation of denatured vaccinia DNA through alkaline sucrose gradients yields an apparent molecular weight greater than twice that of denatured T4 DNA, implying that the complementary strands of vaccinia DNA do not separate upon denaturation. When alkali-denatured vaccinia DNA is ne...
متن کاملMorphology of deoxyribonucleic acid extracted from cores of vaccinia virus.
It has become apparent during the past few years that viral genomes may exist, at some stage of their life cycle, as an endless structure that can for convenience be termed a circle. The deoxyribonucleic acid (DNA) of small viruses such as polyoma appears to exist in this form inside the virion, since it can be extracted as rings a few microns in length (R. Weil and J. Vinograd, Proc. Natl. Aca...
متن کاملDeoxyribonucleic acid-dependent nucleotide phosphohydrolase activity in purified vaccinia virus.
A nucleotide phosphohydrolase (adenosine triphosphatase), which is associated with vaccinia virus cores, has been solubilized and shown to be deoxyribonucleic acid dependent.
متن کاملSequential formation of vaccinia virus proteins and viral deoxyribonucleic acid replication.
In vaccinia virus-infected cell cultures, cellular protein synthesis was inhibited 50% at 2 hr postinfection (PI) and 80 to 90% by 4 hr PI. Input virus was responsible for this inhibition. Five early proteins, coded for by the viral genome, could be detected at 2 to 3 hr PI. Normally, their synthesis did not continue beyond 6 hr PI, at which time synthesis of a different set of proteins began. ...
متن کاملFrog virus 3 deoxyribonucleic acid.
SUMMARY DNA extracted from frog virus 3 has a GC mol fraction of about 0"56. The mol. wt. of the DNA is about Ioo x I@ (97 × IO 6 by neutral sucrose gradient sedimentation; IO2 × IO 6 from renaturation kinetics). Analysis by neutral sucrose sedimentation indicated that the DNA exists as a single heteroduplex in the virus particle. Alkaline sucrose gradient sedimentation indicated that single st...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1970
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.5.3.299-304.1970